human fxii Search Results


fxii deficient plasma  (Innovative Research Inc)
93
Innovative Research Inc fxii deficient plasma
Fxii Deficient Plasma, supplied by Innovative Research Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+fxii/Factor+XII+(FXII)+Deficient+Human+Plasma/us11846641-205-2-14
Average 93 stars, based on 1 article reviews
fxii deficient plasma - by Bioz Stars, 2026-10
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human fxii hufxii  (American Diagnostics)
90
American Diagnostics human fxii hufxii
Infarct volumes and functional outcomes 24 h after focal cerebral ischemia in WT and <t>FXII</t> −/− mice, and in FXII −/− mice infused with human FXII. (A) Representative images of three corresponding coronal sections of WT (left), FXII −/− (middle), and FXII −/− mice reconstituted with human <t>FXII</t> <t>(huFXII,</t> 2 μg/g body weight i.v. 10 min before the MCAO; right) stained with TCC. (B) Brain infarct volumes in WT ( n = 18), FXII −/− ( n = 18), and FXII −/− mice reconstituted with huFXII ( n = 8); **P < 0.01. (C) Neurological Bederson score assessed at day 1 after tMACO for WT ( n = 18), FXII −/− ( n = 18), and huFXII-treated FXII −/− animals ( n = 8); **P < 0.01. n.s., not significant.
Human Fxii Hufxii, supplied by American Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+fxii/human+fxii+hufxii/pmc02118228-78-3-6
Average 90 stars, based on 1 article reviews
human fxii hufxii - by Bioz Stars, 2026-10
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fitc conjugated goat anti-mouse fibrin/fibrinogen serum (nordic immunological laboratories, berks, uk)  (Absolute Biotech)
90
Absolute Biotech fitc conjugated goat anti-mouse fibrin/fibrinogen serum (nordic immunological laboratories, berks, uk)
Infarct volumes and functional outcomes 24 h after focal cerebral ischemia in WT and <t>FXII</t> −/− mice, and in FXII −/− mice infused with human FXII. (A) Representative images of three corresponding coronal sections of WT (left), FXII −/− (middle), and FXII −/− mice reconstituted with human <t>FXII</t> <t>(huFXII,</t> 2 μg/g body weight i.v. 10 min before the MCAO; right) stained with TCC. (B) Brain infarct volumes in WT ( n = 18), FXII −/− ( n = 18), and FXII −/− mice reconstituted with huFXII ( n = 8); **P < 0.01. (C) Neurological Bederson score assessed at day 1 after tMACO for WT ( n = 18), FXII −/− ( n = 18), and huFXII-treated FXII −/− animals ( n = 8); **P < 0.01. n.s., not significant.
Fitc Conjugated Goat Anti Mouse Fibrin/Fibrinogen Serum (Nordic Immunological Laboratories, Berks, Uk), supplied by Absolute Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+fxii/goat+anti+human+fxii+antibody++1+500++gahu+fxii++nordic+mubio++the+netherlands+/pm09453006-55-14-20
Average 90 stars, based on 1 article reviews
fitc conjugated goat anti-mouse fibrin/fibrinogen serum (nordic immunological laboratories, berks, uk) - by Bioz Stars, 2026-10
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antibodies for human fxii  (GeneTex)
90
GeneTex antibodies for human fxii
Penicillin activates the contact system in an <t>FXII-dependent</t> manner. ( A ) Penicillin-induced CSA in standard or FXII-deficient human plasma. 100 μL of plasma was pretreated with 100 μL of penicillin at various concentrations (diluted by Tris buffer: 50 mM Tris–HCl, 0.117 M NaCl, pH 7.8) at 37 °C. Ten minutes later, 100 μL of the chromogenic substrate S-2302 (1.5 mg/mL) was added and further incubated at 37 °C for 30 min. The reaction mix was centrifuged at 3,000 × g for 5 min. Supernatant absorbance was monitored at 405 nm. Kaolin was used as a positive control of the contact system. Buffer alone was included as the negative control. * P < 0.05 and ** P < 0.01 vs. negative control. ( B ) Plasma prototypical FXII level decreased after penicillin treatment. Standard human plasma was incubated with or without 4 KU/mL penicillin at 37 °C for 30 min and analyzed for FXII determination by western blotting. Transferrin was used as the internal reference. Full-length blots and the detailed information of the <t>used</t> <t>antibodies</t> were presented in Supplementary file . ( C ) Penicillin induced BK release in PI-HUVEC. HUVEC were incubated with 10% standard human plasma in the presence of 20 μM Zn 2+ at 37 °C for 1 h. The plasma was removed and the cells were washed twice. PI-HUVEC and non-PI-HUVEC were further incubated with penicillin at 37 °C for 30 min. Supernatant BK was determined by ELISA. ** P < 0.01.
Antibodies For Human Fxii, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+fxii/antibodies+for+human+fxii/pmc07447753-45-2-8
Average 90 stars, based on 1 article reviews
antibodies for human fxii - by Bioz Stars, 2026-10
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monoclonal antibody against human fxii/fxiia having vh/vl sequences of antibody 3f7  (CSL Behring)
90
CSL Behring monoclonal antibody against human fxii/fxiia having vh/vl sequences of antibody 3f7
Penicillin activates the contact system in an <t>FXII-dependent</t> manner. ( A ) Penicillin-induced CSA in standard or FXII-deficient human plasma. 100 μL of plasma was pretreated with 100 μL of penicillin at various concentrations (diluted by Tris buffer: 50 mM Tris–HCl, 0.117 M NaCl, pH 7.8) at 37 °C. Ten minutes later, 100 μL of the chromogenic substrate S-2302 (1.5 mg/mL) was added and further incubated at 37 °C for 30 min. The reaction mix was centrifuged at 3,000 × g for 5 min. Supernatant absorbance was monitored at 405 nm. Kaolin was used as a positive control of the contact system. Buffer alone was included as the negative control. * P < 0.05 and ** P < 0.01 vs. negative control. ( B ) Plasma prototypical FXII level decreased after penicillin treatment. Standard human plasma was incubated with or without 4 KU/mL penicillin at 37 °C for 30 min and analyzed for FXII determination by western blotting. Transferrin was used as the internal reference. Full-length blots and the detailed information of the <t>used</t> <t>antibodies</t> were presented in Supplementary file . ( C ) Penicillin induced BK release in PI-HUVEC. HUVEC were incubated with 10% standard human plasma in the presence of 20 μM Zn 2+ at 37 °C for 1 h. The plasma was removed and the cells were washed twice. PI-HUVEC and non-PI-HUVEC were further incubated with penicillin at 37 °C for 30 min. Supernatant BK was determined by ELISA. ** P < 0.01.
Monoclonal Antibody Against Human Fxii/Fxiia Having Vh/Vl Sequences Of Antibody 3f7, supplied by CSL Behring, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+fxii/monoclonal+antibody+against+human+fxii+fxiia+having+vh+vl+sequences+of+antibody+3f7/us12068729-525-20-2
Average 90 stars, based on 1 article reviews
monoclonal antibody against human fxii/fxiia having vh/vl sequences of antibody 3f7 - by Bioz Stars, 2026-10
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human fxii-deficient plasma  (CSL Behring)
90
CSL Behring human fxii-deficient plasma
Penicillin activates the contact system in an <t>FXII-dependent</t> manner. ( A ) Penicillin-induced CSA in standard or FXII-deficient human plasma. 100 μL of plasma was pretreated with 100 μL of penicillin at various concentrations (diluted by Tris buffer: 50 mM Tris–HCl, 0.117 M NaCl, pH 7.8) at 37 °C. Ten minutes later, 100 μL of the chromogenic substrate S-2302 (1.5 mg/mL) was added and further incubated at 37 °C for 30 min. The reaction mix was centrifuged at 3,000 × g for 5 min. Supernatant absorbance was monitored at 405 nm. Kaolin was used as a positive control of the contact system. Buffer alone was included as the negative control. * P < 0.05 and ** P < 0.01 vs. negative control. ( B ) Plasma prototypical FXII level decreased after penicillin treatment. Standard human plasma was incubated with or without 4 KU/mL penicillin at 37 °C for 30 min and analyzed for FXII determination by western blotting. Transferrin was used as the internal reference. Full-length blots and the detailed information of the <t>used</t> <t>antibodies</t> were presented in Supplementary file . ( C ) Penicillin induced BK release in PI-HUVEC. HUVEC were incubated with 10% standard human plasma in the presence of 20 μM Zn 2+ at 37 °C for 1 h. The plasma was removed and the cells were washed twice. PI-HUVEC and non-PI-HUVEC were further incubated with penicillin at 37 °C for 30 min. Supernatant BK was determined by ELISA. ** P < 0.01.
Human Fxii Deficient Plasma, supplied by CSL Behring, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+fxii/human+fxii+deficient+plasma/10__1074_slash_jbc__m109__045963-103-7-10
Average 90 stars, based on 1 article reviews
human fxii-deficient plasma - by Bioz Stars, 2026-10
90/100 stars
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matched-pair antibody set elisa of human factor xii antigen (fxii-eia)  (CoaChrom Diagnostica GmbH)
90
CoaChrom Diagnostica GmbH matched-pair antibody set elisa of human factor xii antigen (fxii-eia)
Penicillin activates the contact system in an <t>FXII-dependent</t> manner. ( A ) Penicillin-induced CSA in standard or FXII-deficient human plasma. 100 μL of plasma was pretreated with 100 μL of penicillin at various concentrations (diluted by Tris buffer: 50 mM Tris–HCl, 0.117 M NaCl, pH 7.8) at 37 °C. Ten minutes later, 100 μL of the chromogenic substrate S-2302 (1.5 mg/mL) was added and further incubated at 37 °C for 30 min. The reaction mix was centrifuged at 3,000 × g for 5 min. Supernatant absorbance was monitored at 405 nm. Kaolin was used as a positive control of the contact system. Buffer alone was included as the negative control. * P < 0.05 and ** P < 0.01 vs. negative control. ( B ) Plasma prototypical FXII level decreased after penicillin treatment. Standard human plasma was incubated with or without 4 KU/mL penicillin at 37 °C for 30 min and analyzed for FXII determination by western blotting. Transferrin was used as the internal reference. Full-length blots and the detailed information of the <t>used</t> <t>antibodies</t> were presented in Supplementary file . ( C ) Penicillin induced BK release in PI-HUVEC. HUVEC were incubated with 10% standard human plasma in the presence of 20 μM Zn 2+ at 37 °C for 1 h. The plasma was removed and the cells were washed twice. PI-HUVEC and non-PI-HUVEC were further incubated with penicillin at 37 °C for 30 min. Supernatant BK was determined by ELISA. ** P < 0.01.
Matched Pair Antibody Set Elisa Of Human Factor Xii Antigen (Fxii Eia), supplied by CoaChrom Diagnostica GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+fxii/matched+pair+antibody+set+elisa+of+human+factor+xii+antigen++fxii+eia+/pmc06954242-322-11-18
Average 90 stars, based on 1 article reviews
matched-pair antibody set elisa of human factor xii antigen (fxii-eia) - by Bioz Stars, 2026-10
90/100 stars
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antibodies human fxii transferrin  (GeneTex)
90
GeneTex antibodies human fxii transferrin
Penicillin activates the contact system in an FXII-dependent manner. ( A ) Penicillin-induced CSA in standard or FXII-deficient human plasma. 100 μL of plasma was pretreated with 100 μL of penicillin at various concentrations (diluted by Tris buffer: 50 mM Tris–HCl, 0.117 M NaCl, pH 7.8) at 37 °C. Ten minutes later, 100 μL of the chromogenic substrate S-2302 (1.5 mg/mL) was added and further incubated at 37 °C for 30 min. The reaction mix was centrifuged at 3,000 × g for 5 min. Supernatant absorbance was monitored at 405 nm. Kaolin was used as a positive control of the contact system. Buffer alone was included as the negative control. * P < 0.05 and ** P < 0.01 vs. negative control. ( B ) Plasma prototypical FXII level decreased after penicillin treatment. Standard human plasma was incubated with or without 4 KU/mL penicillin at 37 °C for 30 min and analyzed for FXII determination by western blotting. <t>Transferrin</t> was used as the internal reference. Full-length blots and the detailed information of the used antibodies were presented in Supplementary file . ( C ) Penicillin induced BK release in PI-HUVEC. HUVEC were incubated with 10% standard human plasma in the presence of 20 μM Zn 2+ at 37 °C for 1 h. The plasma was removed and the cells were washed twice. PI-HUVEC and non-PI-HUVEC were further incubated with penicillin at 37 °C for 30 min. Supernatant BK was determined by ELISA. ** P < 0.01.
Antibodies Human Fxii Transferrin, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+fxii/antibodies+human+fxii+transferrin/pmc07447753-45-5-8
Average 90 stars, based on 1 article reviews
antibodies human fxii transferrin - by Bioz Stars, 2026-10
90/100 stars
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neutralizing anti–human fxii mab  (QED Bioscience)
90
QED Bioscience neutralizing anti–human fxii mab
Penicillin activates the contact system in an FXII-dependent manner. ( A ) Penicillin-induced CSA in standard or FXII-deficient human plasma. 100 μL of plasma was pretreated with 100 μL of penicillin at various concentrations (diluted by Tris buffer: 50 mM Tris–HCl, 0.117 M NaCl, pH 7.8) at 37 °C. Ten minutes later, 100 μL of the chromogenic substrate S-2302 (1.5 mg/mL) was added and further incubated at 37 °C for 30 min. The reaction mix was centrifuged at 3,000 × g for 5 min. Supernatant absorbance was monitored at 405 nm. Kaolin was used as a positive control of the contact system. Buffer alone was included as the negative control. * P < 0.05 and ** P < 0.01 vs. negative control. ( B ) Plasma prototypical FXII level decreased after penicillin treatment. Standard human plasma was incubated with or without 4 KU/mL penicillin at 37 °C for 30 min and analyzed for FXII determination by western blotting. <t>Transferrin</t> was used as the internal reference. Full-length blots and the detailed information of the used antibodies were presented in Supplementary file . ( C ) Penicillin induced BK release in PI-HUVEC. HUVEC were incubated with 10% standard human plasma in the presence of 20 μM Zn 2+ at 37 °C for 1 h. The plasma was removed and the cells were washed twice. PI-HUVEC and non-PI-HUVEC were further incubated with penicillin at 37 °C for 30 min. Supernatant BK was determined by ELISA. ** P < 0.01.
Neutralizing Anti–Human Fxii Mab, supplied by QED Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+fxii/neutralizing+anti+human+fxii+mab/pmc03158722-30-94-97
Average 90 stars, based on 1 article reviews
neutralizing anti–human fxii mab - by Bioz Stars, 2026-10
90/100 stars
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human fxii  (CellSystems Biotechnologie Vertrieb GmbH)
90
CellSystems Biotechnologie Vertrieb GmbH human fxii
Penicillin activates the contact system in an FXII-dependent manner. ( A ) Penicillin-induced CSA in standard or FXII-deficient human plasma. 100 μL of plasma was pretreated with 100 μL of penicillin at various concentrations (diluted by Tris buffer: 50 mM Tris–HCl, 0.117 M NaCl, pH 7.8) at 37 °C. Ten minutes later, 100 μL of the chromogenic substrate S-2302 (1.5 mg/mL) was added and further incubated at 37 °C for 30 min. The reaction mix was centrifuged at 3,000 × g for 5 min. Supernatant absorbance was monitored at 405 nm. Kaolin was used as a positive control of the contact system. Buffer alone was included as the negative control. * P < 0.05 and ** P < 0.01 vs. negative control. ( B ) Plasma prototypical FXII level decreased after penicillin treatment. Standard human plasma was incubated with or without 4 KU/mL penicillin at 37 °C for 30 min and analyzed for FXII determination by western blotting. <t>Transferrin</t> was used as the internal reference. Full-length blots and the detailed information of the used antibodies were presented in Supplementary file . ( C ) Penicillin induced BK release in PI-HUVEC. HUVEC were incubated with 10% standard human plasma in the presence of 20 μM Zn 2+ at 37 °C for 1 h. The plasma was removed and the cells were washed twice. PI-HUVEC and non-PI-HUVEC were further incubated with penicillin at 37 °C for 30 min. Supernatant BK was determined by ELISA. ** P < 0.01.
Human Fxii, supplied by CellSystems Biotechnologie Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+fxii/human+fxii/pm21929690-56-0-4
Average 90 stars, based on 1 article reviews
human fxii - by Bioz Stars, 2026-10
90/100 stars
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Image Search Results


Infarct volumes and functional outcomes 24 h after focal cerebral ischemia in WT and FXII −/− mice, and in FXII −/− mice infused with human FXII. (A) Representative images of three corresponding coronal sections of WT (left), FXII −/− (middle), and FXII −/− mice reconstituted with human FXII (huFXII, 2 μg/g body weight i.v. 10 min before the MCAO; right) stained with TCC. (B) Brain infarct volumes in WT ( n = 18), FXII −/− ( n = 18), and FXII −/− mice reconstituted with huFXII ( n = 8); **P < 0.01. (C) Neurological Bederson score assessed at day 1 after tMACO for WT ( n = 18), FXII −/− ( n = 18), and huFXII-treated FXII −/− animals ( n = 8); **P < 0.01. n.s., not significant.

Journal: The Journal of Experimental Medicine

Article Title: Targeting coagulation factor XII provides protection from pathological thrombosis in cerebral ischemia without interfering with hemostasis

doi: 10.1084/jem.20052458

Figure Lengend Snippet: Infarct volumes and functional outcomes 24 h after focal cerebral ischemia in WT and FXII −/− mice, and in FXII −/− mice infused with human FXII. (A) Representative images of three corresponding coronal sections of WT (left), FXII −/− (middle), and FXII −/− mice reconstituted with human FXII (huFXII, 2 μg/g body weight i.v. 10 min before the MCAO; right) stained with TCC. (B) Brain infarct volumes in WT ( n = 18), FXII −/− ( n = 18), and FXII −/− mice reconstituted with huFXII ( n = 8); **P < 0.01. (C) Neurological Bederson score assessed at day 1 after tMACO for WT ( n = 18), FXII −/− ( n = 18), and huFXII-treated FXII −/− animals ( n = 8); **P < 0.01. n.s., not significant.

Article Snippet: In some experiments, human FXII (huFXII; American Diagnostics) was injected intravenously immediately before the experiment.

Techniques: Functional Assay, Staining

Penicillin activates the contact system in an FXII-dependent manner. ( A ) Penicillin-induced CSA in standard or FXII-deficient human plasma. 100 μL of plasma was pretreated with 100 μL of penicillin at various concentrations (diluted by Tris buffer: 50 mM Tris–HCl, 0.117 M NaCl, pH 7.8) at 37 °C. Ten minutes later, 100 μL of the chromogenic substrate S-2302 (1.5 mg/mL) was added and further incubated at 37 °C for 30 min. The reaction mix was centrifuged at 3,000 × g for 5 min. Supernatant absorbance was monitored at 405 nm. Kaolin was used as a positive control of the contact system. Buffer alone was included as the negative control. * P < 0.05 and ** P < 0.01 vs. negative control. ( B ) Plasma prototypical FXII level decreased after penicillin treatment. Standard human plasma was incubated with or without 4 KU/mL penicillin at 37 °C for 30 min and analyzed for FXII determination by western blotting. Transferrin was used as the internal reference. Full-length blots and the detailed information of the used antibodies were presented in Supplementary file . ( C ) Penicillin induced BK release in PI-HUVEC. HUVEC were incubated with 10% standard human plasma in the presence of 20 μM Zn 2+ at 37 °C for 1 h. The plasma was removed and the cells were washed twice. PI-HUVEC and non-PI-HUVEC were further incubated with penicillin at 37 °C for 30 min. Supernatant BK was determined by ELISA. ** P < 0.01.

Journal: Scientific Reports

Article Title: Penicillin causes non-allergic anaphylaxis by activating the contact system

doi: 10.1038/s41598-020-71083-x

Figure Lengend Snippet: Penicillin activates the contact system in an FXII-dependent manner. ( A ) Penicillin-induced CSA in standard or FXII-deficient human plasma. 100 μL of plasma was pretreated with 100 μL of penicillin at various concentrations (diluted by Tris buffer: 50 mM Tris–HCl, 0.117 M NaCl, pH 7.8) at 37 °C. Ten minutes later, 100 μL of the chromogenic substrate S-2302 (1.5 mg/mL) was added and further incubated at 37 °C for 30 min. The reaction mix was centrifuged at 3,000 × g for 5 min. Supernatant absorbance was monitored at 405 nm. Kaolin was used as a positive control of the contact system. Buffer alone was included as the negative control. * P < 0.05 and ** P < 0.01 vs. negative control. ( B ) Plasma prototypical FXII level decreased after penicillin treatment. Standard human plasma was incubated with or without 4 KU/mL penicillin at 37 °C for 30 min and analyzed for FXII determination by western blotting. Transferrin was used as the internal reference. Full-length blots and the detailed information of the used antibodies were presented in Supplementary file . ( C ) Penicillin induced BK release in PI-HUVEC. HUVEC were incubated with 10% standard human plasma in the presence of 20 μM Zn 2+ at 37 °C for 1 h. The plasma was removed and the cells were washed twice. PI-HUVEC and non-PI-HUVEC were further incubated with penicillin at 37 °C for 30 min. Supernatant BK was determined by ELISA. ** P < 0.01.

Article Snippet: Antibodies for human FXII and transferrin were from GeneTex Inc. (San Antonio, TX, USA).

Techniques: Clinical Proteomics, Incubation, Positive Control, Negative Control, Western Blot, Enzyme-linked Immunosorbent Assay

Penicillin activates the contact system in an FXII-dependent manner. ( A ) Penicillin-induced CSA in standard or FXII-deficient human plasma. 100 μL of plasma was pretreated with 100 μL of penicillin at various concentrations (diluted by Tris buffer: 50 mM Tris–HCl, 0.117 M NaCl, pH 7.8) at 37 °C. Ten minutes later, 100 μL of the chromogenic substrate S-2302 (1.5 mg/mL) was added and further incubated at 37 °C for 30 min. The reaction mix was centrifuged at 3,000 × g for 5 min. Supernatant absorbance was monitored at 405 nm. Kaolin was used as a positive control of the contact system. Buffer alone was included as the negative control. * P < 0.05 and ** P < 0.01 vs. negative control. ( B ) Plasma prototypical FXII level decreased after penicillin treatment. Standard human plasma was incubated with or without 4 KU/mL penicillin at 37 °C for 30 min and analyzed for FXII determination by western blotting. Transferrin was used as the internal reference. Full-length blots and the detailed information of the used antibodies were presented in Supplementary file . ( C ) Penicillin induced BK release in PI-HUVEC. HUVEC were incubated with 10% standard human plasma in the presence of 20 μM Zn 2+ at 37 °C for 1 h. The plasma was removed and the cells were washed twice. PI-HUVEC and non-PI-HUVEC were further incubated with penicillin at 37 °C for 30 min. Supernatant BK was determined by ELISA. ** P < 0.01.

Journal: Scientific Reports

Article Title: Penicillin causes non-allergic anaphylaxis by activating the contact system

doi: 10.1038/s41598-020-71083-x

Figure Lengend Snippet: Penicillin activates the contact system in an FXII-dependent manner. ( A ) Penicillin-induced CSA in standard or FXII-deficient human plasma. 100 μL of plasma was pretreated with 100 μL of penicillin at various concentrations (diluted by Tris buffer: 50 mM Tris–HCl, 0.117 M NaCl, pH 7.8) at 37 °C. Ten minutes later, 100 μL of the chromogenic substrate S-2302 (1.5 mg/mL) was added and further incubated at 37 °C for 30 min. The reaction mix was centrifuged at 3,000 × g for 5 min. Supernatant absorbance was monitored at 405 nm. Kaolin was used as a positive control of the contact system. Buffer alone was included as the negative control. * P < 0.05 and ** P < 0.01 vs. negative control. ( B ) Plasma prototypical FXII level decreased after penicillin treatment. Standard human plasma was incubated with or without 4 KU/mL penicillin at 37 °C for 30 min and analyzed for FXII determination by western blotting. Transferrin was used as the internal reference. Full-length blots and the detailed information of the used antibodies were presented in Supplementary file . ( C ) Penicillin induced BK release in PI-HUVEC. HUVEC were incubated with 10% standard human plasma in the presence of 20 μM Zn 2+ at 37 °C for 1 h. The plasma was removed and the cells were washed twice. PI-HUVEC and non-PI-HUVEC were further incubated with penicillin at 37 °C for 30 min. Supernatant BK was determined by ELISA. ** P < 0.01.

Article Snippet: Antibodies for human FXII and transferrin were from GeneTex Inc. (San Antonio, TX, USA).

Techniques: Incubation, Positive Control, Negative Control, Western Blot, Enzyme-linked Immunosorbent Assay